Researchers in Germany have found that epithelial-to-mesenchymal transition (EMT), where epithelial cells from placenta are converted into mesenchymal cells and the transitioned cells are transplanted, is beneficial for cardiac regeneration by lowering infract size.
They concluded that EMT enhanced the cardioprotective effects of human AECs.The study will be published in a future issue of Cell Transplantation
but is currently freely available on-line as an unedited early e-pub at: http://www.ingentaconnect.com/content/cog/ct/pre-prints/content-ct1046Roy. The authors noted that AECs have been shown to share characteristics of pluripotent cells that are able to transform into all other kinds of cells, and that their isolation and clinical-grade expansion of AECs is "relatively straightforward." Our hypothesis was that EMT would improve cardiac regeneration capacity of amniotic epithelial cells by increasing their mobility and extracellular matrix modulating capacity," said study corresponding author Dr. Christof Stamm of the Berlin Brandenburg Center for Regenerative Therapies, Berlin, Germany. "Indeed, four weeks after the mice were modeled with myocardial infarction the mice subsequently treated with EMT-AECs were associated with markedly reduced infarct size."According to the researchers, as a result of the EMT process the AECs lost their "cobblestone" structure and acquired a fibroblastoid shape which was associated with a number of biological alterations that ultimately aided their mobility and altered their secretions.
One direct result of the EMT-AEC treatment was that EMT-AEC-treated hearts displayed "better global systolic function and improved longitudinal strain rate in the area of interest." The researchers added that while AECs may be useful in the context of cardiovascular regeneration, it is unclear whether the usefulness requires "actual stemness" or "pluripotency-unrelated secretory mechanisms. "Blood vessel density was increased in both AEC and EMT-AEC-treated hearts and we conclude that EMT enhances the cardioprotective effects of human AECs," they wrote. "This study highlights how AECs are a potentially useful stem cell population whose use can be maximized by a transformation, such as EMT, prior to transplantation" said Dr Amit N. Patel, director of cardiovascular regenerative medicine at the University of Utah and section editor for Cell Transplantation
. "This was demonstrated in a myocardial infarction model but further study is necessary to confirm whether this transformation is relevant for other diseases (and other cell types)".