According to a study by cosmetic surgeons, people who regularly use the treatment for nine years or more have "significantly" higher brows than those who had rarely used the treatment, the study found.
The research found that the brows of patients who repeatedly had the treatments were on average more than 3mm higher than those who did not, long after the short-term effects of the treatment to freeze wrinkles had disappeared, reports Telegraph.
Most of the effects of Botox injections wear off within three to six months, so those who use it to keep wrinkles at bay, pay repeatedly to maintain a smoother complexion.
The research will be presented this week to the annual conference of the British Association of Aesthetic Plastic Surgeons in Chester.
The study is the first to demonstrate that long-term use of Botox can alter the anatomy of the face, reducing the "brow droop" that is considered to be a noticeable aspect of ageing.