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Beta-lactamase Enzyme Makes Bacteria Resistant to Antibiotics

by Colleen Fleiss on February 20, 2018 at 12:53 AM
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Beta-lactamase Enzyme Makes Bacteria Resistant to Antibiotics

Researchers at Stanford University uncover TEM beta-lactamase enzyme's evolution from changing electric fields and resisting antibiotics.

During the Biophysical Society's 62nd Annual Meeting, held Feb. 17-21, 2018, in San Francisco, California, Samuel H. Schneider, a graduate student in Stanford University's Boxer Lab, will present the group's research studying what happens when an enzyme is accelerating reaction and how an enzyme changes over time making it resistant to antibiotics.

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Researchers have been trying to figure out exactly what is happening when an enzyme binds to another molecule and ultimately, how that enzyme becomes resistant to antibiotics. The team of researchers at the Boxer Lab is using an existing technique called the vibrational Stark effect (VSE) in a novel way to measure a molecule's electric field when the enzyme and molecule are attached at different times during the enzyme's evolution to becoming resistant to antibiotics.

The team measured the electric fields generated by a TEM beta-lactamase enzyme attached to two different molecules and the vibration of the chemical bonds in these molecules in the hopes that they will find what makes the enzyme develop a resistance to cephalosporins antibiotics.

Source: Eurekalert
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