Bacterial Proteins Kept in Check by Molecular Chaperone

A new study has found that proteins are minded by chaperones that prevent unwanted interactions among immature clients.

In the research, scientists at the University of Michigan and Howard Hughes Medical Institute have discovered how a protein chaperone called HdeA saves energy while keeping proteins from forming destructive clumps.

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HdeA is known to help protect bacteria like the notorious Escherichia coli from the ravages of stomach acid.

Proteins in disease-causing bacteria like E. coli unfold when they land in stomach acid after being accidentally ingested by humans and other animals.

This unfolding stops the proteins from working and could spell doom for the bacteria if the chaperone HdeA didn't step in.

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HdeA works by binding very tightly to the unfolded proteins while the bacteria are in the stomach. By attaching to the bacterial proteins, the chaperone stops them from tangling like slow-dancing teens, which could kill the bacteria.

The researchers discovered how HdeA could then let go of the unfolded proteins as the bacteria pass into the small intestine so that the proteins refold instead of clumping together.

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"HdeA uses a unique timed-release mechanism. If the proteins were released all at once they would likely clump together, killing the bacteria. What we found instead is that the chaperone HdeA lets go of them gradually, making it more likely that they fold back up into their proper form than clump together," said postdoctoral fellow Tim Tapley, who spearheaded the work.

While most molecular chaperones consume large amounts of cellular energy in order to function, HdeA instead taps energy freely available in its living environment.

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"In this way, HdeA is a bit like a wind powered machine, except that instead of harnessing wind, HdeA uses the energy from pH changes in the surrounding environment as the bacteria move from the acid stomach to the slightly alkaline small intestine," said James Bardwell.

The research is published online in the Proceedings of the National Academy of Sciences.

Source-ANI
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