Haptoglobin (Hp) is a glycoprotein and is produced as an acute phase plasma component and synthesized in many different tissues. A new biosensing platform has been fabricated for the determination of Haptoglobin in human blood.
The glycoprotein has many biochemical and metabolic roles such as hosting defense responses to infection and inflammation, acting as an antioxidant, antibacterial and anti-inflammatory roles, and as an inhibitor of prostaglandin synthesis. Moreover, it is reported that the levels of Hp are elevated in neuromyelitisoptica. Because of the metabolic importance of Hp, its sensitive and selective analysis is also of vital importance to researchers.
In this research, a highly sensitive and disposable Indium Tin Oxide (ITO) based biosensing tool was developed. Featuring the considerable superiorities of ITO substrates, suitability for disposable biosensors, low cost, and repeatability, a novel anti-Hp based biosensor was created with a very low detection limit.
Our research suggests that ITO substrates can be used in other biosensing strategies with high reproducibility and repeatability. Besides, the single frequency impedance technique can be performed in the evaluation of association and dissociation relations of bimolecular interactions, successfully.