How Are Stem Cells Grown In The Laboratory?

Stem cells are grown in a maintained temperature of 37º C in incubators in culture dishes under high humidity.

Different culture medium is used to grow and maintain different types of stem cells.

It is necessary to grow stem cells in an undifferentiated state, and to make the cells differentiate into specialized cells, when required.

Human embryonic stem cells can be grown as small colonies on layers of skin cells in the presence of serum. The skin cells are known as "feeder cells" and together with the serum, provide unknown factors that nourish and support the embryonic stem cells.

The cells are divided into small colonies or single cells and transferred into new culture dish when the large colonies of embryonic stem cells are grown. "Passaging" or transferring is done to grow these cells continuously.

Hematopoietic stem cells are obtained from bone marrow, placenta or umbilical cord blood. As the hematopoietic stem cells differentiate very quickly it is difficult to make them grow in culture medium.

Human bone marrow stem cells are isolated from the bone marrow and grown in culture media supplemented with serum from the blood. Bone marrow cells attach to the bottom of the culture dishes and can grow for several weeks before they differentiate.

Human neural stem cells can grow from fetal or adult brain tissue in culture media. They grow in suspension and they do not need serum from blood.

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JODY2513

i need a stem cell transplant for c.i.d.p. i have been on iv Gamma Glub for 10 years

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Anandkumarsrivastava

I am based in Delhi. How can I avail of your services? how about the costs etc? A.K. Srivastava

reejatharu

we can get totipotent stem cells from human embryos that are created invitro in ART labs.

guest

Hello,
I am a c2/c3 quad on a ventilator. I have been paralyzed for 15 years as a result from fooling with a friend. I writing to find about any type of human spinal cord rejuvenation trials. If so or any type along that line, then please let me know.
Thanks

roheet

contact reelabs at mumbai, reference roheet gupta. they have done such cases sussessfully

Gabriel

I know that Miami college in Florida has been working on this in animals, so they can do this in humans. They have been doing wonders. Give them a call. It might just change your life. Be persistent about it with them. You could also call up Pen State. Their are some wonderful BME people there. M for Medical that is. They might be able to help. Call Florida first for sure. God bless your journey.

Namithann

A fertilised egg has totipotency, or total potential for about four days. Days after fertilisation,the totipotent stem cell divides and then matures to cause more specialised stem cells called pluripotent stem cells.Basically, the pluripotent stem cell can do everything the totipotent one can except for creating an entire organism.

so, how can we take totipotent cells frm a human? is tht practically possible? if we could, it wud be great, isn't it?

Aarex

growing