Sputum Test

Influenza tests

Dr. Nithin Jayan
Medically Reviewed by Dr. Nithin Jayan, MBBS
Last Updated on Jun 04, 2014
Email Print This Page Comment bookmark
Font : A-A+

Test : Influenza tests

Indications : A sputum test for influenza is carried out to confirm the role of influenza virus is establishing flu-like symptoms.

Sputum collection is done early in the morning. The patient must refrain from eating or drinking before the test. It is also essential to brush teeth and rinse mouth well prior to the test. Avoid using antiseptic mouthwash. Sputum may be brought up by deep coughing (expectorated) or it may be or induced with the help of sterile saline or glycerin aerosol. The coughed up sputum is collected into a sterile cup provided by the laboratory.

Although influenza is usually diagnosed on the basis of clinical symptoms, the gold standard laboratory test is the 14 day culture. However the test results take too long and hence, is not often done.

Instead the sputum sample that is collected is subjected to rapid diagnostic tests such as a fluorescent optical immune assay (OIA).Antigens are extracted from the sputum samples and a conjugate added and incubated for 6-7 minutes. It is then washed and blotted. A single drop of substrate was added to the test surface and further incubated for 6-7 minutes.

Physiology : Influenza, commonly known as the “Flu”, is a respiratory epidemic infection caused by a number of viruses. The illness usually last for a week while the symptoms may persist for a couple of weeks longer. The complications from influenza can be severe.

The influenza test is carried out to detect the cause of the flu-like symptoms which include fever, malaise, headache, body pain and chills. The combination of symptoms are non specific and may vary with patients.

Normal Range : Negative

Interpretation :

Positive

If a blue or purple reaction zone appears in the center of the test surface, the result is positive for Influenza A and B.

Negative

If no color change appears, the result is negative for influenza virus A and B.



References : +



Revise This Test    Add New Test

Post a Comment

Comments should be on the topic and should not be abusive. The editorial team reserves the right to review and moderate the comments posted on the site.
Notify me when reply is posted
I agree to the terms and conditions

Lab Test A - Z

A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

Lab Test Search

Premium Membership Benefits

Medindia Newsletters

Subscribe to our Free Newsletters!

Terms & Conditions and Privacy Policy.

Stay Connected

  • Available on the Android Market
  • Available on the App Store