- General Info About Cholera
- What are the Causes of Cholera?
- What are the Symptoms of Cholera?
- How can we Diagnose Cholera?
- How can Cholera be Treated?
- How can we Prevent Cholera?
- Frequently Asked Questions about Cholera
- Latest Publication and Research
How can we Diagnose Cholera?
Cholera can be diagnosed with the help of detailed patient history and stool examination.
A patient history of watery stools especially during an epidemic should raise the suspicion of cholera.
Macroscopic / Gross Examination-
Stools resembles ‘rice water’ and contain mucus
Dark field microscopic examination of stool sample shows rapidly motile V.cholerae bacteria. Epithelial cells are also present in the stool sample.
A stool culture may be done to confirm the presence of V. cholerae. Enrichment is done in alkaline peptone water at pH 8.7 for 6-8 hours incubation.
Typical yellow color colonies appear in thiosulfate-citrate-bile salts-sucrose (TCBS) agar after 18 hours of incubation. Organisms can be confirmed from culture by staining and examining under microscope.
Vibrio cholerae are further identified by slide agglutination tests using anti-O group 1 antiserum and by biochemical reactions.