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Diagnosis of Cholera

Cholera can be diagnosed with the help of detailed patient history and stool examination.

A patient history of watery stools especially during an epidemic should raise the suspicion of cholera.

Stool examination-


Bullet Macroscopic / Gross Examination-

Stools resembles ‘rice water’ and contain mucus

Bullet Microscopic Examination-

Dark field
microscopic examination of stool sample shows rapidly motile V.cholerae bacteria. Epithelial cells are also present in the stool sample.

Bullet Culture-

A stool culture may be done to confirm the presence of V. cholerae. Enrichment is done in alkaline peptone water at pH 8.7 for 6-8 hours incubation.

Typical yellow color colonies appear in thiosulfate-citrate-bile salts-sucrose (TCBS) agar after 18 hours of incubation. Organisms can be confirmed from culture by staining and examining under microscope.

Specific Tests-

Vibrio cholerae
are further identified by slide agglutination tests using anti-O group 1 antiserum and by biochemical reactions.


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Grace is mean to me all the time!!!
kelli.7 Wednesday, May 12, 2010
In Zimbabwe the National Water Authority is failing to supply water to large parts of the urban areas. This has resulted in residences drawing on Boerhole Water and others purchasing from borehole owners. My question is what is the risk of being infected by the Cholera Bacteria especially in schools who use the water to wash with but not to drink?
Gavin Tuesday, November 18, 2008

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