Become a member
Quick Registration
Full Registration Benefits -
Free Homepages for Doctors
Free Homepages for Hospitals
Free Homepages for Medical Conference
Free Homepages for NGO's
Personal Health Record Creation
Access to all Medical Slides
Access to all Jobs & Products Sale
Medindia »
Consumer Health »
Health Information »
Biopsy-Specimen Processing - Permanent Section technique
| Disease Info Index | ||||||||||||||||||||||||||
|
|
Biopsy-Specimen Processing
Page Options
Biopsy - Specimen Processing
4.29
/5
Comments : 1
| |||
|
|||
Text 
|
The fresh specimen is immersed in a liquid called a fixative for several hours (the necessary time depends on the size of the specimen). The fixative, formalin causes the proteins in the cells to denature and become hard and "fixed." Adequate fixation is probably the most important technical aspect of biopsy processing. The fixed specimen is fixed with paraffin wax. The next morning histologist removes the paraffin- impregnated specimen and "embeds" it in a larger block of molten paraffin. This is allowed to solidify by chilling and is set in a cutting machine, called a microtome. The histotech uses the microtome to cut thin sections of the paraffin block containing the biopsy specimen. These delicate sections are floated out on a water bath and picked up on a glass slide. Paraffin wax is dissolved from the tissue block by using solvents. Then the tissues are stained with dyes - Hematoxylin and eosin. The stain combination, casually referred to by pathologists as "H and E" yields pink, orange, and blue sections that make it easier for us to distinguish different parts of cells. Typically, the nucleus of cells stains dark blue, while the cytoplasm stains pink or orange. |
|
|
|
| Share it! |  |
 |
 |
 |
 |
 |
 |
Related Links
Sarcoidosis / Besnier - Boeck disease
Bernie Mac the comedian died of complications arising from Sarcoidosis, a little known immune system ...
Gastric Cancer
The stomach is part of the digestive system. It is located in the upper abdomen, under the ribs on the left side. The upper part of the stomach ...
Biopsy-Introduction
Encyclopedia section of medindia gives meaning to some of the words, for Biopsy, in the ...
Top Ten Facts About Prostate Cancer
This is the most controversial cancer in both treatment and diagnosis. Read interesting facts about this ...
Breast Biopsy
Breast biopsy involves removing a sample of breast tissue to determine whether it is cancerous or ...
Small Intestine Biopsy
Encyclopedia section of medindia gives meaning to some of the words, for small intestine biopsy, in the ...
Bone Marrow Aspiration and Biopsy
Bone marrow biopsy and aspiration is the removal of some bone marrow tissue for diagnosis and management of cancers of blood cells and multiple ...
Comment & Contribute
|
Posted by:
Mr.green(Guest)
Posted on: 08/21/2009 does this technique gives instant report? is it possible to do in same OT premises?
Reply |
Forward
|
