It is crucial to identify people who are at an increased risk of developing colorectal cancer as it is the second most common cause of cancer-related deaths in the Western world, mainly because it is usually diagnosed too late.
Marjaana Pussila, a PhD student in the 'DNA mismatch repair and cancer' group at the University of Helsinki, Finland, explained how her team's work in genetically-engineered mice has helped to reveal the role played by a Western-style diet, rich in fat and low in fiber, vitamin D and folate, in the development of colorectal cancer at the annual conference of the European Society of Human Genetics.
‘Western-style diet seems to be a severe risk factor for the development of a common form of colon cancer called as Lynch Syndrome.’
Interactions between genetic and environmental factors, including diet, are thought to play a crucial role in its development, but the earliest changes in normal colonic mucosa that could lead to early detection and prevention of cancer development remain to be elucidated.Using a mouse model of Lynch syndrome, the most common form of inherited colon cancer, the researchers carried out a long-term diet experiment. The Lynch syndrome mouse carries a mutation in MIh1, a mismatch repair gene, which is one of the main susceptibility genes in the disease. Each time cells divide, DNA has to replicate itself. It is during this process that mistakes can be made, leading to genomic instability and potentially to cancer. Lynch syndrome carriers have inherited one healthy and one mutated mismatch repair gene, and about an 80% risk of developing cancer.
"We wanted to be able to spot cancer-predicting events in the colon mucosa before tumors developed," Ms Pussila explained. "For this reason, we decided to use a mouse model which was already known to have a susceptibility to colon cancer. We reasoned that in mutation carriers requiring just a second hit of the inherited susceptibility gene for malignant transformation, it might be possible to detect the earliest changes, perhaps even preceding the second hit, and distinguish these from alterations occurring later in cancer development."
"Interestingly, our results showed that the gene expression profiles of normal mucosa in those mice that developed colon cancer were very different from those of the mice that did not. This seems to indicate that there is a colon-wide effect of events that predispose to cancer. And the Western-style diet seemed to be a severe risk factor, since 80% of cancers were detected in WD fed mice," said Ms Pussila.
Surprisingly, no typical Lynch syndrome tumor characteristics, such as lack of MIh1 protein or microsatellite instability -- a kind of genetic hyper-mutability -- were detected, although MIh1 gene expression was already severely decreased in the mucosa. This suggests that a decrease in MIh1 gene expression may be sufficient to induce the development of tumors, even where the DNA mismatch repair mechanism that recognizes and corrects mistakes in DNA replication is still operating well enough to avoid microsatellite instability.
"It has been generally assumed that the development of cancer in Lynch syndrome carriers needs an inactivation of both the mismatch repair gene alleles, or pairs, involved, thus causing microsatellite instability. However, our studies have shown that this is not necessarily the case, since a severely decreased amount of MIh1 without loss of the second allele and without microsatellite instability appears to be enough to provoke the development of tumors," said Ms Pussila.
"Now, by studying the gene expression profiles of Mlh1 low mucosa we hope to be able to further identify cancer predisposing changes which may help in the early detection of tumors," concluded Ms Pussila.