A novel stem cell technique promises abundance of cardiomyocytes - key heart cells, say researchers.
A team of Wisconsin scientists has discovered a way to transform human stem cells - both embryonic and induced Pluripotent stem cells - into the critical heart muscle cells by simple manipulation of one key developmental pathway.
The technique promises a uniform, inexpensive and far more efficient alternative method in comparison to the complex bath of serum or growth factors now used to nudge blank slate stem cells to become specialized heart cells.
"Our protocol is more efficient and robust," explains Sean Palecek, the senior author of the new report and a University of Wisconsin-Madison professor of chemical and biological engineering.
"We have been able to reliably generate greater than 80 percent Cardiomyocytes in the final population while other methods produce about 30 percent Cardiomyocytes with high batch-to-batch variability," said Palecek.
According to Palecek, the capability to make the key heart cells in abundance and in a precisely defined way is important because it shows the potential to make the production of large, uniform batches of Cardiomyocytes routine.
The cells are in great demand for research, and increasingly for the high throughput screens used by the pharmaceutical companies to test drugs and potential drugs for toxic effects.
The ability to make the heart cells using induced pluripotent stem cells, which can come from adult patients with diseased hearts, means scientists will be able to more readily model those diseases in the laboratory.
Such cells contain the genetic profile of the patient, and so can be used to reconstruct the disease in the lab dish for study.
Cardiomyocytes are difficult or impossible to obtain directly from the hearts of patients and if obtained, the cells survive only briefly in the lab.
Scientists also have high hopes that one day healthy lab-grown heart cells can be used to substitute the Cardiomyocytes that die as a result of heart disease, the leading cause of death in the United States.
"Many forms of heart disease are due to the loss or death of functioning cardiomyocytes, so strategies to replace heart cells in the diseased heart continue to be of interest," said Timothy Kamp, another senior author of the new PNAS report and a professor of cardiology in the UW School of Medicine and Public Health.
"For example, in a large heart attack up to 1 billion Cardiomyocytes die. The heart has a limited ability to repair itself, so being able to supply large numbers of potentially patient-matched Cardiomyocytes could help," said Kamp.
"These cells will have many applications," said Xiaojun Lian, a UW-Madison graduate student and the lead author of the new study.
The beating cells made using the technique he devised have, so far, been maintained in culture in the lab for six months and remain as viable and stable as the day they were created.
Lian and his colleagues discovered that manipulating a major signaling pathway known as Wnt - turning it on and off at prescribed points in time using just two off-the-shelf small molecule chemicals - is enough to efficiently direct stem cell differentiation to cardiomyocytes.
"The fact that turning on and then off one master signaling pathway in the cells can orchestrate the complex developmental dance completely is a remarkable finding as there are many other signaling pathways and molecules involved," said Kamp.
"The biggest advantage of our method is that it uses small molecule chemicals to regulate biological signals," said Palecek.
"It is completely defined, and therefore more reproducible. And the small molecules are much less expensive than protein growth factors," Kamp added.
This study has been published in the Proceedings of the National Academy of Sciences.