Next Gen PCR Systems may Speed Up Detection of E. Coli

by Kathy Jones on  December 25, 2012 at 7:19 PM Genetics & Stem Cells News
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American researcher Pina Fratamico is currently investigating whether the use of a next-generation real time polymerase chain reaction system can help uncover specific gene targets which indicate the presence of food borne pathogens, including E. coli, a new study published in the journal Frontiers in Microbiology reveals.
 Next Gen PCR Systems may Speed Up Detection of E. Coli
Next Gen PCR Systems may Speed Up Detection of E. Coli

The results show that assays performed using this PCR system are rapid, sensitive, and reliable.

"Testing using these types of systems is faster, easier, and more reproducible than previous methods, and this should increase food safety in the long run. I feel that we could confidently move to these new systems for screening ground beef and other foods for E. coli contamination," says Fratamico, researcher at the USDA Agricultural Research Service in Wyndmoor, Pennsylvania.

Not all E. coli are dangerous, but certain strains produce a potentially dangerous toxin called Shiga toxin. These Shiga toxin-producing E. coli also known as STEC can be found in raw meat and cause serious food poisoning in humans. According the Food Safety and Inspection Service (FSIS) website, in October of this year over, 2,300 pounds of ground beef were recalled due to contamination with STEC.

"Certain groups of STEC have been declared as adulterants by the USDA FSIS, and the availability of rapid and reliable tests for these pathogens is critical so that testing results are available before meat is shipped to restaurants and consumers," explains Fratamico.

The PCR protocol has already been used for some time in the meat industry. The genetic test detects the presence of specific gene targets that indicate the existence of STEC in meat. The new generation of real-time PCR systems, like the GeneDisc from Pall Technologies in France used in this particular study, employ a self-contained unit that standardizes the procedure and tend to be relatively portable and easy to use - offering obvious advantages for both meat processors and inspectors from the industry and government alike.



Source: Eurekalert

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