Scientists might one day be enabled to grow cardiac patches for damaged or diseased hearts from a patient's own stem cells-no matter what age the patient-while avoiding the threat of rejection with the help of a new method which transforms aged stem cells into cells that function like much younger ones.
Stem cell therapies involving donated bone marrow stem cells run the risk of patient rejection in a portion of the population, argues Milica Radisic, Canada Research Chair in Functional Cardiovascular Tissue Engineering at the Institute of Biomaterials and Biomedical Engineering (IBBME) and Associate Professor in the Department of Chemical Engineering and Applied Chemistry at the University of Toronto.
One method of avoiding the risk of rejection has been to use cells derived from a patient's own body. But until now, clinical trials of this kind of therapy using elderly patients' own cells have not been a viable option, since aged cells tend not to function as well as cells from young patients.
Now, Radisic and her co-researcher, Dr. Ren-Ke Li, have come closer to solving this problem, by creating the conditions for a 'fountain of youth' reaction within a tissue culture.
Radisic and Li first create a "micro-environment" that allows heart tissue to grow, with stem cells donated from elderly patients at the Toronto General Hospital.
The cell cultures are then infused with a combination of growth factors-common factors that cause blood vessel growth and cell proliferation-positioned in such a way within the porous scaffolding that the cells are able to be stimulated by these factors.
Dr. Li and his team then tracked the molecular changes in the tissue patch cells.
"We saw certain aging factors turned off," states Li, citing the levels of two molecules in particular, p16 and RGN, which effectively turned back the clock in the cells, returning them to robust and healthy states.
"It's very exciting research," said Radisic, who was named one of the top innovators under 35 by MIT in 2008 and winner of the 2012 Young Engineers Canada award.
Li and Radisic hope to continue their goal to create the most effective environment in which cells from older patients can be given new life.
"We can create much better tissues which can then be used to repair defects such as aneurysms," Li said, as well as repairing damage caused by heart attacks.
The study was recently released in the Journal of the American College of Cardiology, the top journal in the field of cardiovascular medicine.