An End to Bacterial Antibiotic Resistance in Sight

Some drug combinations can stop or even reverse bacterial antibiotic resistance, an American scientist has said.

Harvard researcher Roy Kishony said: "Normally, when clinicians administer a multi-drug regimen, they do so because the drugs act synergistically and speed up bacterial killing."

However, Kishony's laboratory has focused on the opposite phenomenon: antibiotic interactions that have a suppressive effect, namely when the combined inhibitory effect of using the two drugs together is weaker than that of one of the drugs alone.

Kishony and his team identified the suppressive interaction in E. coli, discovering that a combination of tetracycline - which prevents bacteria from making proteins - and ciprofloxacin - which prevents them from copying their DNA - was not as good as slowing down bacterial growth as one of the antibiotics (ciprofloxacin) by itself.

Kishony notes that this suppressive interaction can halt bacterial evolution, because any bacteria that develop a resistance to tetracycline will lose its suppressive effect against ciprofloxacin and die off; therefore, in a population the bacteria that remain non-resistant become the dominant strain.

While such a weakened antibiotic combination is not great from a clinical standpoint, the Kishony lab is using this discovery to set up a drug screening system that could identify novel drug combinations that could hinder the development of resistance but still act highly effectively.

He said: "Typical drug searches look for absolute killing effects, and choose the strongest candidates.

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"Our approach is going to ask how these drugs affect the competition between resistant versus sensitive bacterial strains."

To develop such a screen, Kishony and his group first had to figure how this unusual interaction works.

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Kishony said: "Fast growing bacteria like E. coli are optimized to balance their protein and DNA activity to grow and divide as quickly as the surrounding environment allows.

"However, when we exposed E. coli to the ciprofloxacin, we found that their optimization disappeared."

He went on: "We expected that since the bacteria would have more difficulty copying DNA, they would slow down their protein synthesis, too.

"But they didn't; they kept churning out proteins, which only added to their stress."

However, once they added the tetracycline and protein synthesis was also reduced in the E. coli, they actually grew better than before.

They then confirmed the idea that production of ribosomes - the cell components that make proteins - is too high under DNA stress by engineering E. coli strains that have fewer ribosomes than regular bacteria.

While these mutants grew a more slowly in normal conditions, they grew faster under ciprofloxacin inhibition of DNA synthesis.

Kishony believes their preliminary work on the development of a screen for drugs that put resistance in a disadvantage looks promising, and hopes that it would lead to the identification of novel drugs that select against resistance.

Kishony discussed his work at the American Society for Biochemistry and Molecular Biology's annual meeting, titled "Driving backwards the evolution of antibiotic resistance," on April 25.

Source-ANI
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