Researchers Reveal Polyclonality of BRAF Mutations in Acquired Melanocytic Nevi

by Rajashri on  September 17, 2009 at 3:46 PM Genetics & Stem Cells News
RSS Email Print This Page Comment bookmark
Font : A-A+

 Researchers Reveal Polyclonality of BRAF Mutations in Acquired Melanocytic Nevi
A new brief communication published online September 14 in the Journal of the National Cancer Institute reveals that the polyclonality of BRAF mutations in melanocytic nevi suggests that mutation of BRAF may not be an initial event in melanocyte transformation.

To test the polyclonality of BRAF mutations, Minoru Takata, M.D., of the department of dermatology at the Shinshu University School of Medicine in Matsumoto, Japan, and colleagues separated approximately 50 single cells from each of 13 melanocytic nevi by using immunomagnetic beads or by laser-capture microdissection. They were then subjected to single-cell polymerase chain reaction and sequencing to determine BRAF mutations. In another experiment, BRAF and a neighboring single-nucleotide polymorphism were simultaneously amplified from nevi of four patients who were heterozygous for the polymorphism.

Although BRAF mutations were always found among nevus cells, cells that contained only wild-type BRAF predominated in nine of 13 nevi. When BRAF was sequenced from both alleles of four patients heterozygous for an adjacent polymorphism, both alleles harbored BRAF mutations.

"[P]olyclonality of BRAF mutations in the lesions of acquired melanocytic nevi suggests an alternative to the view that BRAF mutation is an initial event in melanocytic neoplasia," the authors write.



Source: Eurekalert
RAS

Post a Comment

Comments should be on the topic and should not be abusive. The editorial team reserves the right to review and moderate the comments posted on the site.
Notify me when reply is posted
I agree to the terms and conditions

More News on:

McArdle Disease 

News A - Z

A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

News Search

Medindia Newsletters

Subscribe to our Free Newsletters!

Terms & Conditions and Privacy Policy.

Find a Doctor

Stay Connected

  • Available on the Android Market
  • Available on the App Store

Facebook

News Category

News Archive