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Research Says UV Light Controls Antibodies, Improves Biosensors

by Sheela Philomena on  November 4, 2011 at 4:39 PM Research News   - G J E 4
A novel way to increase the number of right-side-up antibodies in the well-established molecule detection process - using light has been identified by researchers from the University of Naples "Federico II" and the Second University of Naples in Italy.
 Research Says UV Light Controls Antibodies, Improves Biosensors
Research Says UV Light Controls Antibodies, Improves Biosensors
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WASHINGTON, Oct. 31—From detecting pathogens in blood samples to the study of protein synthesis, Quartz Crystal Microbalance (QCM) sensors have many uses in modern biology. In this technique, antibodies anchored to gold electrodes on a piece of quartz crystal act like the "hooks" on the sticky side of a Velcro strap, grabbing molecules of interest as they pass by. The more molecule-sensing antibodies on the surface of the sensor, the more sensitive the QCM device's detection capabilities.

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Unfortunately, some of the antibodies typically anchor themselves to the gold plate "hook"-side-down, rendering them useless as bio-receptors and dampening the sensor's sensitivity.In a paper recently published in the open-access journal Biomedical Optics Express, the team of scientists irradiated antibodies with ultra-short pulses of ultraviolet (UV) light. The UV light is absorbed by the amino acid tryptophan, which breaks the disulfide bridges holding parts of the antibody together and causes a particular part of the amino acid cysteine, called a thiol group, to become exposed at the tail end of the antibody. Because thiol groups are more strongly attracted to the gold electrodes than other parts of the antibody, the bottom sides of these irradiated antibodies become much more likely to adhere to the gold electrodes than the "hook" ends. Using this method, the researchers were able to more than double the sensitivity of the QCM device, opening up new possibilities for research using this type of sensor, the researchers say.

Source: Eurekalert
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Alert! This photonic technology has been previously published by Neves-Petersen et al. in 2006!! Their work reached the front page of Protein Science. Original Paper: Protein Sci. 2006 Feb;15[2]:343-51. Photonic activation of disulfide bridges achieves oriented protein immobilization on biosensor surfaces. Neves-Petersen MT, Snabe T, Klitgaard S, Duroux M, Petersen SB. NanoBiotechnology Group, Department of Physics and Nanotechnology, Aalborg University, Aalborg, Denmark.
science_fairness Thursday, September 27, 2012

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