New clues about the cause of brain cell death in neurodegenerative disorders such as Parkinson's, Alzheimer's and Huntington's diseases have been discovered by scientists at Sanford-Burnham Medical Research Institute (Sanford-Burnham).
The researchers say that the answer in some cases is the untimely transfer of a gaseous molecule (known as nitric oxide, or NO) from one protein to another.
Advertisement"We and other researchers have shown that NO and related molecules can contribute to either nerve cell death or nerve cell survival. However, these new findings reveal that NO can actually jump from one protein to another in molecular pathways that lead to cellular suicide," said Stuart A. Lipton, senior author of the study and director of the Del E. Web Center for Neuroscience, Aging and Stem Cell Research at Sanford-Burnham.
"Now that we have this molecular clue to the cause of nerve cell death in Parkinson's, Alzheimer's, and Huntington's diseases, we can figure out how to use it to better diagnose and treat these diseases." Lipton added.
In this study, Lipton and his colleagues, led by Tomohiro Nakamura, found that NO-like molecules are transferred from caspases, proteins that normally initiate cell death, to XIAP, a protein that normally inhibits cell death. In other words, caspases pass NO to XIAP like a 'hot potato.'
This process occurs by a chemical reaction known as transnitrosylation. When XIAP is left holding NO, the result is a double whammy for brain cells, since cells are programmed to self-destruct when either XIAP has NO attached to it or when caspases don't. Hence, both brain cell-destroying events occur at the same time.
The researchers then found that XIAP holding the NO 'hot potato' was much more common in brains of human patients with neurodegenerative diseases than in normal brains, solidifying their suspicion that this protein modification leads to cell damage.
To calculate which protein is more likely to end up with the NO 'hot potato,' caspases or XIAP, the researchers created a new version of the Nernst equation - a 19th century mathematical equation taught in every general chemistry class. This power of prediction might allow doctors to diagnose neurodegenerative disorders like Parkinson's or Alzheimer's disease earlier.
"We are currently analyzing cerebrospinal fluid and brain tissue from Parkinson's, Alzheimer's and other patients to determine if we can use the NO-tagged proteins as biomarkers for the disease," Lipton said.
The study has been published in the July 30 issue of Molecular Cell.
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