An improved a strain of E. coli that makes it grow faster has been developed by a University of Illinois researcher.
"The average person hears E. coli and thinks of E. coli 0157:H7, a microorganism that causes horrific food poisoning, but we've developed a strain of E. coli that is suitable for mass production of high-quality DNA that could be used in vaccines or gene therapy," said Yong-Su Jin.
AdvertisementAccording to Jin, industrial strains of E. coli have already been used to produce such diverse products as insulin for diabetics; enzymes used in laundry detergent, and polymer substitutes in carpets and plastic.
"E. coli bacteria have contributed vastly to our scientific understanding of genes, proteins, and the genome as a model system of biology research.
"E. coli DH5a has been so popular that scientists have used it to perform most recombinant DNA techniques. But its slow growth has been a critical weakness," Jin noted.
Because scientists had used random mutagenesis, they weren't sure where the mutation that caused the slow growth had occurred.
"We learned that the scientists had unintentionally weakened a key enzyme in a gene in the nucleotide biosynthesis pathway. When we reversed this mutation, the modified strain grew as quickly as other types of E. coli used in industry while retaining the traits that make it useful in scientific experiments," he said.
The beauty of the new strain lies in the purity and abundance of the DNA that it contains, which makes it a candidate for use in important biotechnological applications, he said.
"For example, to make DNA vaccines and perform gene therapy, we need DNA that is extremely clean and pure. The E. coli strain we have developed is an excellent candidate to deliver this high-quality genetic material in large quantities," he added.