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Human Embryonic Stem Cells Created from 4-cell Embryo
The researchers believe that their findings might make it possible in the future to produce hESC lines at an earlier stage without destroying the embryo.
Blastomeres are formed in the very early stages of embryonic development. About 24 hours after fertilisation the egg divides into two cells.
The division into four cells occurs after 48 hours. After 96 hours, at the morula stage, the fertilized egg has divided four to five times. During this time the size of the embryo does not increase, so the cells become smaller and smaller and they are strongly attached to each other which makes them more difficult to manipulate.
At this early stage important decisions are taken: inner cells will become the foetus (including germ cells) and outer cells will become trophoblast (the outermost layer of the embryo that attaches to the wall of the uterus and serves as a nutritive pathway).
There was, until now, uncertainty about which stage of early development the blastomeres ceased to be totipotent, i.e. able to develop into all cell types of the body.
"Previously, scientists have been able to derive hESC lines at the 8-cell stage, but success rates were variable and it was necessary to culture them by mixing with established hESC lines," said Dr. Van de Velde
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More News on: Stem Cells - Cord Blood, Stem Cells - Fundamentals, Parkinsons Disease Surgical Treatment, Genetics and Stem Cells, Bone Marrow Transplantation, Tissue Engineering and Regenerative Medicine, Stem Cells
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