New research suggests that chronic pain, which most often occurs without an apparent trigger, may result from the inadvertent reprogramming of more than 2,000 genes in the peripheral nervous system.
Mayo Clinic researchers think that the finding could ultimately lead to 'transcription therapy', which would employ drugs that kill pain by correcting the activity of specific genes.
The researchers focused on nerve cells suspected to be involved in pain: dorsal root ganglion neurons of the peripheral nervous system in rodent models. They performed high-throughput sequencing of hundreds of millions of mRNA molecules, the messengers of gene activity.
Powerful computer science was required to sort through the many pieces of information (50 base-pair long mRNA sequence "reads") assembling the complicated genomic puzzle.
The resulting picture revealed a number of surprises, among them 10,464 novel exons (sections of the genome involved in creating proteins) and some 400 gene candidates described for the first time in the study.
Furthermore, detailed building plans for thousands of spliced mRNA were mapped.
"Using this new approach offers greater sensitivity, dynamic range and more efficient unbiased genetic mapping compared to the previous microarray-based methods and may be an efficient new approach to a wide array of problems in neuroscience research," says Andreas Beutler, Mayo Clinic oncologist and co-author on the study.
The findings appear in the current issue of the journal Genome Research.