Gene Expression Changes may be Responsible for Clinical Complications Following Major Trauma

Inflammatory complications following major trauma are associated with the changes in gene expression, shows study. These complications put patients’ at higher risk of developing serious or fatal complications such as major organ failure.

These findings, from a study conducted by John Storey of Princeton University, USA and colleagues as part of the US-based Inflammation and the Host Response to Injury Large-Scale Collaborative Research Program and published in this week's issue of the journal PLoS Medicine, will help scientists understand why some patients develop inflammatory complications of trauma, which are responsible for more than half of long-term trauma-related deaths, while others do not. The study will also help to identify new drug targets for future therapy.

Accident and Trauma Care - Golden Hour - Taking Care - Don’t
Accident and trauma care is an important public health challenge that requires immediate attention. Injury (trauma) is the leading cause of death for all age groups under the age of 44.

The authors followed 168 patients for up to 28 days after they experienced blunt-force trauma and used a molecular biology tool (a DNA microarray) to determine gene expression patterns in leukocytes (a type of immune system cell) collected from multiple blood samples taken from each patient during the first few days after injury. Then the authors categorized patients according to the degree of each patient's clinical inflammatory response (based on a measure of multi-organ failure called the Marshall score) and developed a statistical method to associate the early changes in gene expression with clinical outcomes.

The authors found that a quarter of genes, including some genes responsible for initiating and driving immune and inflammatory responses, showed early expression changes between 40 to 80 hours post-injury that were particularly strongly associated with longer-term complications. These expression changes also provided the strongest discrimination between patient clinical outcomes.

However, because gene expression was measured for total blood leukocytes isolated from peripheral blood samples, some of the differential expression changes identified could be confounded by changes in individual leukocyte subpopulations.

Head Trauma - First Aid and Emergency Treatment Guide
A quick and simple First Aid guide on how to administer treatment for Head Trauma.

The authors say: "These findings potentially provide the most comprehensive picture of the gene expression response to trauma to date, thereby demonstrating the power of moving beyond candidate gene studies of this clinical condition."

They continue: "The expression variation at the genomic level that we have characterized among patients may help to provide a more comprehensive set of drug targets and a means to identify relevant subsets of patients for which these may be effective."

Gene Expression Clustering Linked to Glaucoma Protection
Glaucoma, the second most common cause of blindness in the US. Researchers have examined the gene expression patterns in the retina and optic nerve of mice that leads to glaucoma.

The authors add: "Additionally, the quantitative approach we have introduced is potentially applicable to future genomics studies of rapidly progressing clinical conditions."

Source-Eurekalert

Alterations in "Inflammation-Control" Kidney Genes Causes More Organ Failure
Kidney damage sets off complications in patients, spreading organ failure throughout their bodies due to alterations in kidney-based genes that sabotage inflammation-control.