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Designer E. Coli to Produce Flavonoids as a Solution for Ageing, Cancer and Obesity
"Ultimately, we want to be able to take a designed E. coli off of the shelf and drop into it the enzymes that constitute a particular biosynthetic pathway in order to make exactly the product we want," said Dr. Mattheos A. G. Koffas, assistant professor of chemical and biological engineering in the School of Engineering and Applied Sciences, and leader of the research team.
He said that microbial biosynthesis reduces or eliminates the need for petrochemical sources, elevated temperatures, toxic heavy metal catalysts, extremes of acidity and dangerous solvents through the use of specially adapted bacteria, specialized enzymes and natural feedstock.
The researcher revealed that his team was using can facilitate chemical reactions, which are difficult to accomplish through conventional chemistry such as chiral syntheses.
"We are finding out how we can actually 'train' microbial systems to produce high yields of chemicals to be used as pharmaceuticals and to make production processes more efficient, less expensive and more environmentally friendly," Koffas said.
He revealed that his team had produced 400 milligrams of flavonoids per litre of cell culture, far above the next highest yield of about 20 milligrams per litre produced by other microbial synthesis efforts. It had been reported in Applied and Environmental Microbiology in June, he added.
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