DNA Analysis Using Nanoparticles

by Sheela Philomena on  October 10, 2011 at 2:23 PM Research News   - G J E 4
A novel process has been invented by University of Arkansas researcher that preforms DNA analysis thousands times faster than traditional methods.
 DNA Analysis Using Nanoparticles
DNA Analysis Using Nanoparticles

This development could contribute to many areas of health care and law enforcement, including diagnosing and treating disease, developing and testing new vaccines and forensic identification.

Donald K. Roper, associate professor of chemical engineering, explained that the ultimate goal of his research is to develop a credit-card-sized device to be used in a doctor's office or at a crime scene to quickly analyze samples of DNA.

To analyze DNA, scientists must often make a tiny sample large enough to work with. To do this, they use a process called polymerase chain reaction, or PCR.

Roper's process, which he developed while working at the University of Utah, uses gold nanoparticles to increase the efficiency of the chain reaction.

During the reaction, strands of DNA are heated and cooled in cycles. When the samples are heated, the two strands of a DNA double helix come apart, and when the temperature is lowered, an enzyme called polymerase zips each strand to other, complementary strands, forming two new DNA helixes.

These copies are then heated and cooled again, doubling each time until the desired amount of DNA has been produced.

Roper's method reduces the time involved in these cycles from minutes to milliseconds, which means that a DNA sample could be analysed within minutes rather than hours.

Roper has patented the process.

Source: ANI

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