American tissue engineers have come out with a new method to assemble artificial tissues.
Although tissue engineering holds promise for building new organs to replace damaged livers, blood vessels and other body parts, the one major hurdle researchers faced until now was to get cells grown in a lab dish to form 3-D shapes instead of flat layers.
AdvertisementHowever, scientists at the MIT-Harvard Division of Health Sciences and Technology (HST) have developed a new way to overcome that challenge - by encapsulating living cells in cubes and arranging them into 3-D structures, just as a child constructs buildings out of blocks.
The new technique, dubbed "micromasonry," employs a gel-like material that acts like concrete, binding the cell "bricks" together as it hardens.
The tiny cell bricks hold potential for building artificial tissue or other types of medical devices, according to Jennifer Elisseeff, associate professor of biomedical engineering at Johns Hopkins University, who was not involved in the research.
She said: "They're very elegant and have a lot of flexibility in how you grow them.
"It's very creative."
To obtain single cells for tissue engineering, researchers have to first break tissue apart, using enzymes that digest the extracellular material that normally holds cells together.
However, once the cells are free, it's difficult to assemble them into structures that mimic natural tissue microarchitecture.
Some scientists have successfully built simple tissues such as skin, cartilage or bladder on biodegradable foam scaffolds.
Ali Khademhosseini, assistant professor of HST, said: "That works, but it often lacks a controlled microarchitecture.
"You don't get tissues with the same complexity as normal tissues."
The HST researchers built their "biological Legos" by encapsulating cells within a polymer called polyethylene glycol (PEG), which has many medical uses.
Their version of the polymer is a liquid that becomes a gel when illuminated, so when the PEG-coated cells are exposed to light, the polymer hardens and encases the cells in cubes with side lengths ranging from 100 to 500 millionths of a meter.
Once the cells are in cube form, they can be arranged in specific shapes using templates made of PDMS, a silicon-based polymer used in many medical devices.
Both template and cell cubes are coated again with the PEG polymer, which acts as a glue that holds the cubes together as they pack themselves tightly onto the scaffold surface.
After the cubes are arranged properly, they are illuminated again, and the liquid holding the cubes together solidifies. When the template is removed, the cubes hold their new structure.
Former HST postdoctoral associate Javier Gomez Fernandez and Khademhosseini used this method to build tubes that could function as capillaries, potentially helping to overcome one of the most persistent problems with engineered organs - lack of an immediate blood supply.
Gomez Fernandez, now a postdoctoral fellow at Harvard, said: "If you build an organ, but you can't provide nutrients, it is going to die."
They hope their work could also lead to a new way to make artificial liver or cardiac tissue.
Other researchers have developed a technique called organ printing to create complex 3-D tissues, but that process requires a robotic machine that is not in widespread use.
The new technique does not require any special equipment.
Gomez Fernandez said: "You can reproduce this in any lab.
"It's very simple."
The study has appeared published online in the journal Advanced Materials.