Drug Name : Methotrexate
Indication :Widely used anticancer drug acting as an antimetabolite in DNA synthesis.
Physiology :Methotrexate is an antimetabolite that combines with dihydrofolate reductase and interferese with the synthesis of tetrahydrofolic acid necessary for DNA synthesis.
Normal Values :Normal values depend upon the drug therapy.
Critical Values :>2.27 µg/mL (> 5 µmol/L)
Test Method :Enzyme immunoassay, Radio immunoassay, HPLC
Side effects :Combination of salicylate administration increases incidence of toxicity, due to diminished renal tubular excretion.